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All Journal HAYATI Journal of Biosciences Jurnal Fitopatologi Indonesia Jurnal Teknologi Dan Industri Pangan Jurnal Akuakultur Indonesia Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis Berkala Perikanan Terubuk BIOTROPIA - The Southeast Asian Journal of Tropical Biology Current Biochemistry Journal Jurnal Kelautan Tropis Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Marinade Jurnal Teknologi Pertanian Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan JURNAL PERIKANAN TROPIS Asian Journal of Aquatic Sciences COJ (Coastal and Ocean Journal) Barakuda 45
Kustiariyah Tarman
Departemen Teknologi Hasil Perairan, Fakultas Perikanan Dan Ilmu Kelautan, IPB University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Library & Information Science Medicine & Pharmacology Social Sciences Veterinary

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Journal : Jurnal Teknologi Dan Industri Pangan

 1 
FRAKSINASI PROTEIN KAPANG LAUT Xylaria psidii KT30 DAN SITOTOKSISITASNYA TERHADAP SEL HeLa [Fractionation of Proteins of Marine Fungus Xylaria psidii KT30 and their Cytotoxicity against HeLa Cells] Mita Gebriella Inthe; Kustiariyah Tarman; Mega Safithri
Jurnal Teknologi dan Industri Pangan Vol. 25 No. 1 (2014): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (578.397 KB) | DOI: 10.6066/jtip.2014.25.1.39

Abstract

FRAKSINASI PROTEIN KAPANG LAUT Xylaria psidii KT30 DAN SITOTOKSISITASNYA TERHADAP SEL HeLa[Fractionation of Proteins of Marine Fungus Xylaria psidii KT30 and their Cytotoxicity against HeLa Cells]Mita Gebriella Inthe1), Kustiariyah Tarman1)* dan Mega Safithri2)1) Departemen Teknologi Hasil Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor, Bogor2) Departemen Biokimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor, Bogor Diterima 22 Juli 2013 / Disetujui 03 Maret 2014ABSTRACT Cervical cancer is the most common cause of death for Indonesian women after human breast cancer. One of the efforts of cancer treatment is the utilization of natural compounds. One of the microorganisms having the potential as anticancer agent is endophytic fungi. Endophytic fungi from the marine habitat can be isolated from sea weeds, sea grasses, sponges, and mangroves. Xylaria psidii KT30, a marine fungus used in this study was isolated from red seaweed Kappaphycus alvarezii. Xylaria psidii KT30 was cultivated in potato dextrose broth medium for nine days at room temperature 27-29°C in shaking condition. This study aimed to obtain protein fractions from X. psidii KT30 and determine their toxicity againt Chang and HeLa cells. The fractionation process was conducted using DEAE Sephadex A-50 column chromatography and the toxicity was determined by Brine Shrimp Lethality Test (BSLT). The metabolites excreted in the culture broth was extracted using 90% of ammonium sulphate. The extract was then tested for their toxicity against HeLa and Chang cells by Microculture Tetrazolium Technique (MTT) assay.The results revealed that LC50 of the protein extract of X. psidii KT30 was 104.95 ppm and IC50 was 69.9 ppm. Based on the National Cancer Institute (NCI), this value showed moderate cytotoxicity against HeLa cells.
AKTIVITAS ANTIBAKTERI PROTEIN KAPANG Xylaria psidii KT30 TERHADAP Escherichia coli DAN Bacillus subtilis [Antibacterial Activity of Protein Fungus Xylaria psidii KT30 Escherichia coli and Bacillus subtilis] Aris Munandar; A. Zaenal Mustopa; Kustiariyah Tarman; Tati Nurhayati
Jurnal Teknologi dan Industri Pangan Vol. 25 No. 2 (2014): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (397.636 KB) | DOI: 10.6066/jtip.2014.25.2.146

Abstract

Previous research shows that extracellular protein of an algicolous fungus Xylaria psidii KT30 inhibited Bacillus pumilus, Listeria sp., Salmonella typhi, Staphylacoccus aureus, and Pseudomonas sp. with an average clear zone diameter of 7 mm. To enhance the potent antibacterial activity of extracellular protein from Xylaria psidii KT30, the present research demonstrated fungal growth optimization and purification of its secreted extracellular protein. The fungal growth optimization was performed following NaCl concentration and harvest day variations. The protein was precipitated using ammonium sulphate at 60%-90% saturation range and was purified through gel chromatography filtrationusing Sephadex G-50, eluted with 30% aq methanol. The active fraction possesing antibacterial activity was then determined resulting supernatant, pellet, and protein fraction.The fungal was optimally obtained after 15 days cultivation using freshwater.The highest protein yield was 1.67%, resulted over 90% saturation. Fractions 11 and 12 were the most active against Escherichia coli dan Bacillus subtiliswith clear zone diameter of 8 mm.Three bands of those fractions were detected through SDS-PAGE analysis, revealing molecular weights of 23.42, 20.09, and 14.33 kDa.  
Co-Authors - - nurjanah A. Alhana A. Zaenal Mustopa Abdul Basir Agus Sabdono Agus Trianto Ahmad Fauzan Lubis anak agung putu ayu puspita negara Anisa Gianti Zhafira Apon Zaenal Mustopa Aulia Andhikawati Azwin Apriandi Azwin Apriandi Azwin Apriandi Bugi Ratno Budiarto Bustami Ibrahim Desniar . Dinamella Wahjuningrum dita aguistina barus Dwi Safitri Efi Toding Tondok Ekowati Chasanah Endar Marraskuranto Hana Nurullita Prestisia Hartoyo Notonegoro Hartoyo Notonegoro Hawis H Madduppa Heder Djamaludin HEDI INDRA JANUAR Hedi Indra Januar Hendra Susanto Hendra Susanto Imra Imra Iriani Setyaningsih Irzal Effendi Jae-Kwan Hwang Mada Triandala Sibero Mada Triandala Sibero Mega Safithri Mega Safithri MEGA SAFITHRI Meydia Meydia Meydia Meydia Meydia Mita Gebriella Inthe Muhammad Nursid Muhammad Nursid Munandar, Aris Munti Yuhana Nabila Ukhty Neni Widowati Novriyandi Hanif Nur Asmasari Syam Nur Syafiqoh Nurjanah - - Nurjanah Nurjanah Nurul Mutia Putram Nurzakiah Nurzakiah Nurzakiah Nurzakiah Ocky Karna Radjasa Pipih Suptijah Prayoga Pannindriya Prayoga Pannindrya Purwantiningsih Sugita putri, R Marwita Sari Putri, Raja Marwita Sari Putriana Sari Sirait Putu Kristiani Kalontong R. Marwita Sari Putri R. Marwita Sari Putri Rita Sahara Riviani Riviani Sari Putri, R. Marwita Sihono Sihono Sri Hariati Sri Purwaningsih Susanto, Hendra Swestri Utami TATI NURHAYATI Taufik Indarmawan Teuku Reza Efianda Tiara Ulfa Bachtiarini Uju Vepryany Oktaviarty Vitriyanna Mutiara Yuhendri Wahyu Ramadhan Widodo Widodo Woro Hastuti Setyantini Yogiara Yogiara Yulia Oktavia Yusro Nuri Fawzya Zafira Khoirunnisa